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2 Jul 2015??????? ????? ???????? (????? 100??+???.-??????????? 100??), Soprodal NV BE. 1 098,20 ???. ??????. ??????? ??????? 200??, Medgenix Benelux.????? ???????: ????????? ??????? ? ????????. ????? ??????? ? ????, ??????, Easy Glam - ????? ???????, ????? ??? ????? (Easy glam) Selective

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The best results were obtained using HPTLC silicagel 60F 254 plates with aluminium base of 20 x 20 cm, with a bed thickness of 0. We follow the protocol cited in [5 ], ????????? ??????? ?????????????????. Each sample extract and standards for the most common bile acids for mammals are spotted on the plates with the use of a capillary tube: LCA, TCA, GCA, CA, CDCA, DCA, DHCA, GCDCA and CHOL, ????????????????? ??????? ?????????. Bile acid standard stock solutions are prepared in methanol at a concentration of 0. We tested the use of different sample (75, 90, 105, 120, 150 and 180 l) and standard (7. As development solvent we use a mixture of toluene:aceticacid:water in a proportion of 5:5:1. We first saturate the mobile phase with water vapour and then we extract the rest of the liquid water. Finally, we were able to differentiate all Xenarthra species through their fecal bile acid patterns, by HPLC, ????? ??? ????????? ???????? ?????????. This study is of great relevance because is the first one in reporting HPLC as an ecological tool for the identification of wild-collected mammal feces. Moreover, it has proved to be a relatively simple method, without large preparation and derivatization steps, achieving resolution and identification of most of the compounds in a short time, ? ??? ???????? ??????, ??? ?????? ???????? ?. Conclusion As a non-invasive method, the analysis of feces is a fundamental tool for field work in ecological studies, not only to identify the presence of certain species in a particular area [94 ], but also for studying threatened species or animals difficult to observe and trap. For the identification, the original fecal shape must be maintained; however, as several factors can corrode it through time, visual identification is not always reliable.

 

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